A quantitative estimate of flash-induced Ca2+- and Na+-influx and Na+/Ca2+-exchange in blowfly calliphora photoreceptors

The flash-induced Ca(2+)- and Na(+)-influx and Na+/Ca(2+)-exchange activity in blowfly Calliphora photoreceptors were investigated. The change in membrane potential, induced by a bright flash, was intracellularly measured in vivo. Based on a biophysical photoreceptor model, the Na(+)- and Ca(2+)-currents and concentration changes were determined from the first transient depolarization phase of the photoreceptor response. The activity of Na+/Ca(2+)-exchange was determined from the after depolarization phase. It appeared that the Na(+)-influx by Na+/Ca(2+)-exchange is about twice that through light-activated channels, suggesting a substantial contribution of Na+/Ca(2+)-exchange to Na(+)-regulation.